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KMID : 0380020080230060513
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 6 p.513 ~ p.518
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Anti-wrinkle Activity of β-carotene Extracted & Purified from Recombinant Escherichia coli
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Abstract
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This paper described the extraction/purification of β-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified β-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used.
However, extraction from wet-cell cake resulted in 2-fold higher amount of β-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of β-carotene corresponding to 61.2% of recovery. The formation and separation of β-carotene crystal improved the purity. 633 mg of β -carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of β-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. 1.7 μM of β-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of β-carotene. This indicates that highly purified β-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.
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KEYWORD
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Recombinant Escherichia coil, β-carotene, Extraction, Anti-wrinkle activity, Functional cosmetics
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